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endothelial cell growth medium 2 supplement pack  (PromoCell)


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    Structured Review

    PromoCell endothelial cell growth medium 2 supplement pack
    Endothelial Cell Growth Medium 2 Supplement Pack, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endothelial+cell+growth+medium+2+supplement+pack/Endothelial+Cell+Growth+Medium+2/pmc12644823-30-40-47
    Average 97 stars, based on 197 article reviews
    endothelial cell growth medium 2 supplement pack - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: 3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors.
    Article Snippet: .. Human umbilical vein endothelial cells (HUVECs; Lonza, C2519A) were cultured at 37 °C in 5% CO2 on gelatincoated cell culture dishes in Endothelial Cell Growth Medium 2 (EGM2) consisting of Endothelial Cell Basal Medium 2 (EBM; PromoCell, C-22211) and Endothelial Cell Growth Medium 2 Supplement Pack (PromoCell, C-39211). .. The final concentration of each component was 2% FCS, 5 ng/ml EGF, 10 ng/ml FGF2, 20 ng/ml IGF1, 0.5 ng/ml VEGF, 1 μg/ml ascorbic acid, 22.5 μg/ml heparin, 0.2 μg/ml hydrocortisone, and 1% PenicillinStreptomycin (P/S; Biosera, XC-A4122).

    Article Title: Aptamer based spatiotemporally controlled growth factor patterning for tunable local microvascular network formation in engineered tissues
    Article Snippet: Seq., 46-nt, DNA, IDT), 5’Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), human VEGF ELISA kit (RAB0507-1KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stem cells (hMSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), L-abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H+L) cross-adsorbed goat anti-rabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H+L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H+L) highly cross-adsorbed goat anti-mouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Article Title: Leukocyte-mimetic liposomes possessing leukocyte membrane proteins pass through inflamed endothelial cell layer by regulating intercellular junctions.
    Article Snippet: Nanoparticles such as liposomes have been applied for the treatment of various diseases such as cancer and inflammatory diseases by utilizing the enhanced permeability and retention effect.. However, their entry into inflammation sites is still limited since passive delivery of nanoparticles is often hampered by the presence of endothelial barriers.. As leukocytes can pass through the inflamed endothelium via utilizing membrane protein functions, we hypothesized that incorporating leukocyte membrane proteins onto liposomal membranes may impart leukocyte-mimicking functions to liposomes, allowing for their adherence to and active passage through the inflamed endothelium.

    Article Title: 3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors
    Article Snippet: .. Human umbilical vein endothelial cells (HUVECs; Lonza, C2519A) were cultured at 37 °C in 5% CO 2 on gelatin-coated cell culture dishes in Endothelial Cell Growth Medium 2 (EGM2) consisting of Endothelial Cell Basal Medium 2 (EBM; PromoCell, C-22211) and Endothelial Cell Growth Medium 2 Supplement Pack (PromoCell, C-39211). .. The final concentration of each component was 2% FCS, 5 ng/ml EGF, 10 ng/ml FGF2, 20 ng/ml IGF1, 0.5 ng/ml VEGF, 1 μg/ml ascorbic acid, 22.5 μg/ml heparin, 0.2 μg/ml hydrocortisone, and 1% Penicillin-Streptomycin (P/S; Biosera, XC-A4122).

    Article Title: Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues.
    Article Snippet: Seq., 46-nt, DNA, IDT), 5′Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), Fluoro-Max dyed blue aqueous fluorescent particles (2 μm diameter) (B0200, Thermo Scientific), human VEGF ELISA kit (RAB0507-1 KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stromal cells (MSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), L-abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1–155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25 ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H + L) cross-adsorbed goat antirabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H + L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H + L) highly cross-adsorbed goat antimouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (ab206887, abcam), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Article Title: Weak Electric Current Treatment to Artificially Enhance Vascular Permeability in Embryonated Chicken Eggs.
    Article Snippet: Cell Culture The human umbilical vein endothelial cells (HUVECs) were purchased from PromoCell GmbH (Heidelberg, Germany). .. HUVECs were cultured in endothelial growth medium (EGM-2; PromoCell GmbH) composed of endothelial basal medium (EBM-2; PromoCell GmbH), 100 U/mL penicillin-100 μg/mL streptomycin (Gibco, MA, U.S.A.), and endothelial cell growth medium-2 supplement pack (PromoCell GmbH). ..

    Article Title: Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues
    Article Snippet: Seq., 46-nt, DNA, IDT), 5′Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), Fluoro-Max dyed blue aqueous fluorescent particles (2 μm diameter) (B0200, Thermo Scientific), human VEGF ELISA kit (RAB0507-1 KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stromal cells (MSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), l -abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1–155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25 ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H + L) cross-adsorbed goat anti-rabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H + L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H + L) highly cross-adsorbed goat anti-mouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (ab206887, abcam), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Aptamer based spatiotemporally controlled growth factor patterning for tunable local microvascular network formation in engineered tissues
    Article Snippet: Seq., 46-nt, DNA, IDT), 5’Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), human VEGF ELISA kit (RAB0507-1KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stem cells (hMSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), L-abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H+L) cross-adsorbed goat anti-rabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H+L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H+L) highly cross-adsorbed goat anti-mouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Article Title: Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues.
    Article Snippet: Seq., 46-nt, DNA, IDT), 5′Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), Fluoro-Max dyed blue aqueous fluorescent particles (2 μm diameter) (B0200, Thermo Scientific), human VEGF ELISA kit (RAB0507-1 KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stromal cells (MSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), L-abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1–155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25 ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H + L) cross-adsorbed goat antirabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H + L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H + L) highly cross-adsorbed goat antimouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (ab206887, abcam), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Article Title: Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues
    Article Snippet: Seq., 46-nt, DNA, IDT), 5′Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), Fluoro-Max dyed blue aqueous fluorescent particles (2 μm diameter) (B0200, Thermo Scientific), human VEGF ELISA kit (RAB0507-1 KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stromal cells (MSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), l -abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1–155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25 ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H + L) cross-adsorbed goat anti-rabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H + L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H + L) highly cross-adsorbed goat anti-mouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (ab206887, abcam), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Recombinant:

    Article Title: Aptamer based spatiotemporally controlled growth factor patterning for tunable local microvascular network formation in engineered tissues
    Article Snippet: Seq., 46-nt, DNA, IDT), 5’Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), human VEGF ELISA kit (RAB0507-1KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stem cells (hMSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), L-abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H+L) cross-adsorbed goat anti-rabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H+L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H+L) highly cross-adsorbed goat anti-mouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Article Title: Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues.
    Article Snippet: Seq., 46-nt, DNA, IDT), 5′Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), Fluoro-Max dyed blue aqueous fluorescent particles (2 μm diameter) (B0200, Thermo Scientific), human VEGF ELISA kit (RAB0507-1 KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stromal cells (MSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), L-abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1–155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25 ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H + L) cross-adsorbed goat antirabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H + L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H + L) highly cross-adsorbed goat antimouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (ab206887, abcam), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Article Title: Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues
    Article Snippet: Seq., 46-nt, DNA, IDT), 5′Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), Fluoro-Max dyed blue aqueous fluorescent particles (2 μm diameter) (B0200, Thermo Scientific), human VEGF ELISA kit (RAB0507-1 KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stromal cells (MSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), l -abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1–155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25 ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H + L) cross-adsorbed goat anti-rabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H + L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H + L) highly cross-adsorbed goat anti-mouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (ab206887, abcam), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Double Staining:

    Article Title: Aptamer based spatiotemporally controlled growth factor patterning for tunable local microvascular network formation in engineered tissues
    Article Snippet: Seq., 46-nt, DNA, IDT), 5’Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), human VEGF ELISA kit (RAB0507-1KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stem cells (hMSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), L-abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H+L) cross-adsorbed goat anti-rabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H+L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H+L) highly cross-adsorbed goat anti-mouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Article Title: Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues.
    Article Snippet: Seq., 46-nt, DNA, IDT), 5′Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), Fluoro-Max dyed blue aqueous fluorescent particles (2 μm diameter) (B0200, Thermo Scientific), human VEGF ELISA kit (RAB0507-1 KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stromal cells (MSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), L-abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1–155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25 ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H + L) cross-adsorbed goat antirabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H + L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H + L) highly cross-adsorbed goat antimouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (ab206887, abcam), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..

    Article Title: Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues
    Article Snippet: Seq., 46-nt, DNA, IDT), 5′Alexa Fluor 488 modified complementary sequence (Fluoro-Comp. .. Seq., 46-nt, DNA, IDT), nuclease free water (11-04-02-01, IDT), Fluoro-Max dyed blue aqueous fluorescent particles (2 μm diameter) (B0200, Thermo Scientific), human VEGF ELISA kit (RAB0507-1 KT, Sigma Aldrich), glutaraldehyde solution (340855, Sigma Aldrich), human umbilical vein endothelial cells (HUVECs, C2519A, Lonza), human mesenchymal stromal cells (MSC, PT-2501, Lonza), α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS, F7524, Sigma), GlutaMaxTM supplement (35050061, Gibco), penicillin-streptomycin (pen/strep, 15140-122, Gibco), l -abscorbic acid (A8960, Sigma Aldrich), trypsin-EDTA 0.25% (+phenol red, 25200072, Gibco), vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), GibcoTM FGF-Basic AA 1–155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2, C-22211, PromoCell), endothelial cell growth medium 2 supplement pack (EGM 2, C-39211, PromoCell), PDMS silicone elastomer (2401673921, Sylgard), live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25 ML, Sigma Aldrich), TritonTM X-100 (T8787, Sigma Aldrich), Phalloidin-Atto 488 (49409, Sigma Aldrich), InvitrogenTM Alexa FluorTM 647 Phalloidin (A22287, Fisher Scientific), InvitrogenTM hoechst 33342 (H1399, Fisher Scientific), monoclonal anti-actin, α-smooth muscle antibody (mouse, A2547, Sigma Aldrich), anti-von Willebrand factor antibody (rabbit, F3520, Sigma Aldrich), human VE-Cadherin antibody (mouse, MAB9381, R&D systems), IgG (H + L) cross-adsorbed goat anti-rabbit, Alexa Fluor® 488, InvitrogenTM (A11008, Fisher Scientific), IgG (H + L) cross-adsorbed goat anti-mouse, Alexa Fluor® 488, InvitrogenTM (A11001, Fisher Scientific), IgG (H + L) highly cross-adsorbed goat anti-mouse, Alexa Fluor® 594, InvitrogenTM (A11032, Fisher Scientific), Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (ab206887, abcam), Corning® 96 well black polystyrene microplate (CLS3603, Sigma), Corning® Costar® ultra-low attachment well plates were used for all cell culture experiments (CLS3473, CLS3474, Sigma). ..



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